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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
30/08/2022 |
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Data da última atualização: |
30/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, E. C. A. de; SILVA, B. P.; PORTO, C.; PILAU, E. J.; MENEZES, C.; FLACH, A. |
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Afiliação: |
EDINEIDE CRISTINA ALEXANDRE DE SOUZA, UNIVERSIDADE FEDERAL DE RORAIMA; BEATRIZ PAES SILVA, UNIVERSIDADE ESTADUAL DE MARINGÁ; CARLA PORTO, MS BIOSCIENCE INCUBADORA TECNOLOGICA DE MARINGÁ; EDUARDO JORGE PILAU, UNIVERSIDADE ESTADUAL DE MARINGÁ; CRISTIANO MENEZES, CNPMA; ADRIANA FLACH, UNIVERSIDADE FEDERAL DE RORAIMA. |
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Título: |
Molecular network-guided chemical profile and mass spectrometry, volatile compounds, and antimicrobial activity of Scaptotrigona depilis propolis. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Rapid Communications in Mass Spectrometry, V. 36, N. 19, e9348, 202. |
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ISSN: |
0951-4198 |
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DOI: |
https://doi.org/10.1002/rcm.9348 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Rationale Propolis has a great diversity in its composition due to numerous factors; therefore, each study is an important contribution to the knoFwledge of its composition and biological action. The objective of this study was to determine the chemical profile and biological activity of propolis produced by Scaptotrigona depilis. Methods Extracts with 70% ethanol (EPE70) and with cereal alcohol (CAPE) were elaborated, and then characterized using UHPLC-ESI(+)-MS/MS. Volatile compounds were extracted and then characterized using gas chromatography mass spectrometry (GC?MS). In addition, antimicrobial activities were verified against resistant strains. Results The volatile compounds of propolis predominantly consist of sesquiterpenes. Using the exploratory metabolomic approach, compounds of different classes were putatively identified in the ethanolic extracts, of which the most representative were terpenes, and some of the sesquiterpenes identified among the volatiles were also detected. The extracts were shown to be active against Escherichia coli and Staphylococcus aureus bacteria with a minimum inhibitory concentration (MIC) of 0.5 and 1.0 mg?mL?1, respectively. Conclusions The molecular network approach proved to be determining the chemical profile of S. depilis propolis rapidly and accurately, and led to the identification of lipophilic compounds. The identification of compounds using GC?MS and UHPLC-ESI(+)-MS/MS is complementary and useful for the characterization of propolis. MenosAbstract: Rationale Propolis has a great diversity in its composition due to numerous factors; therefore, each study is an important contribution to the knoFwledge of its composition and biological action. The objective of this study was to determine the chemical profile and biological activity of propolis produced by Scaptotrigona depilis. Methods Extracts with 70% ethanol (EPE70) and with cereal alcohol (CAPE) were elaborated, and then characterized using UHPLC-ESI(+)-MS/MS. Volatile compounds were extracted and then characterized using gas chromatography mass spectrometry (GC?MS). In addition, antimicrobial activities were verified against resistant strains. Results The volatile compounds of propolis predominantly consist of sesquiterpenes. Using the exploratory metabolomic approach, compounds of different classes were putatively identified in the ethanolic extracts, of which the most representative were terpenes, and some of the sesquiterpenes identified among the volatiles were also detected. The extracts were shown to be active against Escherichia coli and Staphylococcus aureus bacteria with a minimum inhibitory concentration (MIC) of 0.5 and 1.0 mg?mL?1, respectively. Conclusions The molecular network approach proved to be determining the chemical profile of S. depilis propolis rapidly and accurately, and led to the identification of lipophilic compounds. The identification of compounds using GC?MS and UHPLC-ESI(+)-MS/MS is complementary and useful for the characteriz... Mostrar Tudo |
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Palavras-Chave: |
Abelha mandaguari. |
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Thesagro: |
Abelha Brasileira; Análise Química; Espectrometria; Própolis. |
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Thesaurus Nal: |
Antibacterial properties; Honey bees; Mass spectrometry; Scaptotrigona; Stingless bees; Terpenoids; Volatile compounds. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 02585naa a2200349 a 4500
001 2145862
005 2022-08-30
008 2022 bl uuuu u00u1 u #d
022 $a0951-4198
024 7 $ahttps://doi.org/10.1002/rcm.9348$2DOI
100 1 $aSOUZA, E. C. A. de
245 $aMolecular network-guided chemical profile and mass spectrometry, volatile compounds, and antimicrobial activity of Scaptotrigona depilis propolis.$h[electronic resource]
260 $c2022
520 $aAbstract: Rationale Propolis has a great diversity in its composition due to numerous factors; therefore, each study is an important contribution to the knoFwledge of its composition and biological action. The objective of this study was to determine the chemical profile and biological activity of propolis produced by Scaptotrigona depilis. Methods Extracts with 70% ethanol (EPE70) and with cereal alcohol (CAPE) were elaborated, and then characterized using UHPLC-ESI(+)-MS/MS. Volatile compounds were extracted and then characterized using gas chromatography mass spectrometry (GC?MS). In addition, antimicrobial activities were verified against resistant strains. Results The volatile compounds of propolis predominantly consist of sesquiterpenes. Using the exploratory metabolomic approach, compounds of different classes were putatively identified in the ethanolic extracts, of which the most representative were terpenes, and some of the sesquiterpenes identified among the volatiles were also detected. The extracts were shown to be active against Escherichia coli and Staphylococcus aureus bacteria with a minimum inhibitory concentration (MIC) of 0.5 and 1.0 mg?mL?1, respectively. Conclusions The molecular network approach proved to be determining the chemical profile of S. depilis propolis rapidly and accurately, and led to the identification of lipophilic compounds. The identification of compounds using GC?MS and UHPLC-ESI(+)-MS/MS is complementary and useful for the characterization of propolis.
650 $aAntibacterial properties
650 $aHoney bees
650 $aMass spectrometry
650 $aScaptotrigona
650 $aStingless bees
650 $aTerpenoids
650 $aVolatile compounds
650 $aAbelha Brasileira
650 $aAnálise Química
650 $aEspectrometria
650 $aPrópolis
653 $aAbelha mandaguari
700 1 $aSILVA, B. P.
700 1 $aPORTO, C.
700 1 $aPILAU, E. J.
700 1 $aMENEZES, C.
700 1 $aFLACH, A.
773 $tRapid Communications in Mass Spectrometry, V. 36, N. 19, e9348, 202.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Origem |
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Cutter |
Registro |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
04/02/2019 |
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Data da última atualização: |
07/02/2020 |
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Autoria: |
SILVA, S. S. da; FELDMANN, P. R.; SPERS, R. G.; BAMBINI, M. D. |
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Afiliação: |
SHEILA SERAFIM DA SILVA, FEA/USP; PAULO ROBERTO FELDMANN, FEA/USP; RENATA GIOVINAZZO SPERS, USP; MARTHA DELPHINO BAMBINI, CNPTIA, Unicamp. |
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Título: |
Analysis of the process of technology transfer in public research institutions: the Embrapa Agrobiology case. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: SEMINÁRIOS EM ADMINISTRAÇÃO, 20., 2017, São Paulo. Anais... [S.l.: s.n.], 2017. p. 1-16. |
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ISSN: |
2177-3866 |
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Idioma: |
Inglês |
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Notas: |
SemeAd 2017. |
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Conteúdo: |
The literature on TT in IPPs and TT in agricultural research and technological innovation has been revised. Following this, the Embrapa Agrobiology Case and the methodological procedures used in conducting this study were presented. Subsequently, the results and the discussion of these were presented regarding the prospection of technology demand, as well as the process of technology transfer occurs. Finally, final considerations were made, appropriating conclusions, followed by limitations and propositions for the continuation of this study. |
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Palavras-Chave: |
Embrapa Agrobiologia. |
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Thesagro: |
Inovação; Transferência de Tecnologia. |
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Thesaurus NAL: |
Innovation adoption; Technology transfer. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/191983/1/Bambini-Semead-2017.pdf
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Marc: |
LEADER 01315nam a2200229 a 4500
001 2105402
005 2020-02-07
008 2017 bl uuuu u00u1 u #d
022 $a2177-3866
100 1 $aSILVA, S. S. da
245 $aAnalysis of the process of technology transfer in public research institutions$bthe Embrapa Agrobiology case.$h[electronic resource]
260 $aIn: SEMINÁRIOS EM ADMINISTRAÇÃO, 20., 2017, São Paulo. Anais... [S.l.: s.n.], 2017. p. 1-16.$c2017
500 $aSemeAd 2017.
520 $aThe literature on TT in IPPs and TT in agricultural research and technological innovation has been revised. Following this, the Embrapa Agrobiology Case and the methodological procedures used in conducting this study were presented. Subsequently, the results and the discussion of these were presented regarding the prospection of technology demand, as well as the process of technology transfer occurs. Finally, final considerations were made, appropriating conclusions, followed by limitations and propositions for the continuation of this study.
650 $aInnovation adoption
650 $aTechnology transfer
650 $aInovação
650 $aTransferência de Tecnologia
653 $aEmbrapa Agrobiologia
700 1 $aFELDMANN, P. R.
700 1 $aSPERS, R. G.
700 1 $aBAMBINI, M. D.
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Embrapa Agricultura Digital (CNPTIA) |
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